Studies on the treponemal immobilization test.
نویسنده
چکیده
Since the introduction of a serological test for syphilis by Wassermann, Neisser, and Bruck (1906), an immense amount of work has been carried out to improve tests for the detection of " reagin" in the sera of patients with syphilis. The relationship of reagin to the T. pallidum is still not settled with certainty and, although tests for the detection of this substance have reached a high degree of specificity and sensitivity, the need for a practical test which would demonstrate antibodies clearly directed against the causative organism of syphilis has long been felt. The existence of such antibodies has been known for a long time, as early as 1912 Touraine described a method for demonstrating the agglutination of T. pallidum by syphilitic serum (Touraine, 1912). Other methods, such as those described by Turner (1939), have needed the use of animals for the demonstration of protective antibodies against T. pallidum and are therefore not suitable for routine laboratory use. The reports by Nelson (1948), and Nelson and Mayer (1949) of a relatively simple technique by which a treponemal immobilizing antibody, clearly distinct from reagin, could be demonstrated in syphilitic sera aroused great interest. The preliminary work suggested that the test was highly specific, reactions only being given by sera from patients with syphilis and other closely allied treponematoses (yaws, bejel, pinta). In a paper on; he techniques of measurement of immobilizing antibody, Nelson and Diesendruck (1951) laid down certain criteria of positivity. Sera which, under test conditions (at a final dilution of 1 in 10, incubation at 350 C. for 18 hrs) gave 0 to 19 per cent. specific immobilization (S.I.) were classed as negative, from 20 to 49 per cent. S.I. as doubtful, and 50 per cent. or more S.I. as positive. These limits were set from practical experience with the test (Nelson, personal communication) and the data on which they were based were not published. They
منابع مشابه
Studies on the treponemal immobilization test. III. Use of the TPI as a verification test in suspected latent syphilis.
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عنوان ژورنال:
- The British journal of venereal diseases
دوره 30 3 شماره
صفحات -
تاریخ انتشار 1954